Journal: International Journal of Molecular Sciences

Article Title: Effects of Periostracum Cicadae on Cytokines and Apoptosis Regulatory Proteins in an IgA Nephropathy Rat Model

doi: 10.3390/ijms19061599

Figure Lengend Snippet: TLR4, MCP-1, IgA, and caspase-3 expression in rat kidney tissues. ( a ) TLR4, IgA, MCP-1, and caspase-3 expression levels were analyzed by Western blotting. ( b ) Quantitative analysis of TLR4, IgA, MCP-1, and caspase-3 protein levels. A: Healthy, normal controls; B: IgAN model group; C: prednisone acetate group; D: tripterygium glycoside tablet group; E–G: treatment group that received 0.5, 1, and 2 g/kg periostracum cicadae. Data represent the mean ± standard deviation (** p < 0.01 vs model group).

Article Snippet: Rabbit anti-TLR4 polyclonal antibody (Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-IgA polyclonal antibody (Boster Biological Technology), rabbit anti-MCP-1 polyclonal antibody (Boster Biological Technology), and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG antibody (Boster Biological Technology) were used in the detection of the corresponding target proteins.

Techniques: Expressing, Western Blot, Standard Deviation

Journal: Science Advances

Article Title: Immunosuppressive macrophages determine the effect of cellular senescence on tumor progression

doi: 10.1126/sciadv.adx2988

Figure Lengend Snippet: ( A ) Tumor samples from mice treated with vehicle or AP21967 at 9 weeks were homogenized, and the levels of the indicated cytokines were analyzed using a cytokine array. The log 2 FC (AP21967/vehicle) for each cytokine is presented. ENA-78, epithelial-derived neutrophil-activating peptide 78; GROα, growth-regulated oncogene-alpha. ( B ) Levels of CCL2 and CXCL1 were quantified by ELISA from the same tumor homogenates as in (A). ( C ) UMAP plots showing the expression of the most up-regulated cytokines (CCL2, CCL3, and CCL7) and their receptors (CCR1, CCR2, and CCR5) in different cell populations. Relevant cell clusters are indicated. N, neutrophils; MØ, macrophages; E, endothelial cells; F, fibroblasts. ( D ) Flow cytometry analysis of myeloid cells purified from tumors obtained from SuSe mice treated with vehicle or AP21967. We identified TAMs and cells positive for the myeloid marker CD11B and for the macrophage marker F4/80. ( E ) The same cells as in (D) were cultured for 24 hours—in the presence of vehicle or anti-CCL2—and the presence of CCL2 in the conditioned medium was determined by ELISA. ( F ) Schematic representation of the specificities of receptors for CCL2, CCL3, and CCL7. ( G ) Flow cytometry analysis of CCR2 in the same cells as in (D). FSH-H, forward scatter height.

Article Snippet: For selective CCL2 blocking, 4-week-old PyMT/SuSe mice were treated every 3 days with anti-CCL2 antibody (#BE0185, BioXCell,) at a concentration of 2 μg/g of body weight.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Purification, Marker, Cell Culture

Journal: Journal of Occupational Medicine and Toxicology (London, England)

Article Title: Lipopolysaccharide induced inflammation in the perivascular space in lungs

doi: 10.1186/1745-6673-3-17

Figure Lengend Snippet: A-F: H&E histology demonstrating the PVI 12 h after instillation of 25 μg LPS (A, B) . A massive alveolitis can be seen (C). The leukocytes are mainly ED1 positive monocytes/macrophages (D). MCP-1 expression is demonstrated on the apical epithelium and on the endothelium and most of the leukocytes as well (E control, F MCP-1).

Article Snippet: Sections were incubated with primary antibodies against rat monocyte/macrophage (1:75; ED1, Serotec Inc. NC, USA) or rat MCP-1 (1:300; Torrey Pines Biolabs, Inc. TX, USA), followed by appropriate horseradish peroxidase(HRP)-conjugated secondary antibodies (1:100; Dako cytomation, ON, Canada).

Techniques: Expressing